https://www.fynchbio.com/home daily 1.0 2018-07-15 https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1502769998593-R3ACJ79PQ9V3S8KG895V/Lima01.jpg Home https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1502758688005-N6T4FRCHYJNSYN6PS8IB/image-asset.jpeg Home https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1502799861961-YU7H4FI2VFX4JDUPR8KJ/icon.png Home https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1502758644173-RFSDNFQHYI5DB32EE4KD/image-asset.jpeg Home https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1502747285706-4A6ET8GU9IXQKPKBDGAD/IMG_7249.jpg Home https://www.fynchbio.com/tutorials daily 0.75 2018-07-01 https://www.fynchbio.com/tutorials/2018/7/1/building-a-smart-sleeve-for-continuous-culture monthly 0.5 2018-07-01 https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530466215486-BQ340OG9KLCWJ8SG5PWT/IMG_0089.jpg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530402064329-V6ZZFUV9F7FSYAYWEH0F/build_ss3 Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530452893681-9QKNVILSUIBMO4BOEZQZ/IMG_0077.jpg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530400376815-3T1GZNQVBC0Q6NG9MT90/IMG_0059.jpg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530466878786-EG1H7KFNFVGWHDGPY4E4/image-asset.jpeg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530466803898-DPRT5I0QRJLGW1BGU56U/IMG_0119.jpg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530466277915-O6J0RQ8JSNTBURBQVZN3/image-asset.jpeg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530466404012-I6T6793OXCA3IOKP9VZI/image-asset.jpeg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530465453745-ZJ5MW34U5ZVFWWYRFL1C/Screen+Shot+2018-07-01+at+1.08.26+PM.png Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530474601925-T6VGM3A1FVZTKGDHPT8Z/Screen+Shot+2018-07-01+at+3.48.56+PM.png Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530451641033-BL5UNGZ3HGRY2EP6MHV4/Screen+Shot+2018-06-30+at+7.07.56+PM.png Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530466942317-YB708NZHGYLP4L6GIS8S/image-asset.jpeg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530302173553-OK7L2E30HV1O9FCUKFMF/IMG_0043.jpg Tutorials - Build your own Smart Sleeve https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1530465745433-G5S46NZG0Y2VI7P4M1HZ/IMG_0081.jpg Tutorials - Build your own Smart Sleeve https://www.fynchbio.com/tutorials/2017/12/19/odcal-gen1 monthly 0.5 2018-01-26 https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516969577200-ZT9XI3TFN2EY808L1BIR/IMG_7926.JPG Tutorials - Calibrating Optical Density One of 16 different optical density concentrations for full calibration curve. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516981424421-A06R7YIEUR4HS2BNR9N4/position_1.png Tutorials - Calibrating Optical Density https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516984277342-IC98TN7XXH6UETDN24L4/image-asset.png Tutorials - Calibrating Optical Density https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516935647933-UJKPJY8MSYLABUT652NY/IMG_9847.JPG Tutorials - Calibrating Optical Density Saturated culture with 16 assembled vials filled with water (20 mL) https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516972974060-398S01QE0NXU6V3N68WN/Screen+Shot+2018-01-25+at+2.59.19+PM.png Tutorials - Calibrating Optical Density Enter measured OD600 values: Values from step 3 should be entered from smallest to largest values in the cal_curve matrix. There should be 16 values entered in here ranging from 0 to .8. These values will be used to generate the calibration file and graph. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516981608781-7VRYVO7S06G39VXOQ3E0/Screen+Shot+2018-01-25+at+3.03.34+PM.png Tutorials - Calibrating Optical Density https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516972945572-A82ZYWJONJR1CN0AIZYV/Screen+Shot+2018-01-25+at+2.58.43+PM.png Tutorials - Calibrating Optical Density LED Power Settings: The power of the LED can be tuned to optimize for the optical density measurements in eVOLVER. Therefore, it is critical that during the calibration, the correct LED settings are used. The default set up will use a power setting of 2125. Enter a string of 16 comma-separated values in OD_config to determine LED power. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516984070948-OAMM2F88VAW6RKA80OJW/IMG_1396.jpg Tutorials - Calibrating Optical Density If difficult to remove from the Smart Sleeve, a small rod or stick can help remove the vial. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516935721125-6M23Z90AUNJ7BKE9NQHX/IMG_2829.JPG Tutorials - Calibrating Optical Density https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516985121835-ROW4Q28O1KLRCDOT7YXZ/Screen+Shot+2018-01-26+at+11.43.15+AM.png Tutorials - Calibrating Optical Density One of 16 calibration curves generated. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516969828832-EZHYPLP72AK09FT2WNR6/IMG_8459.JPG Tutorials - Calibrating Optical Density Diluting concentrated cells into water. Extra long pipette tips can be used to load small volumes of cells. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516934927601-0CP7WVQMHT9IOJL8PPEY/IMG_0066.JPG Tutorials - Calibrating Optical Density Vial cap inserts to block light during calibration. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516982305733-179QDETYV3P1FKKAHI9D/position_2.png Tutorials - Calibrating Optical Density Shift vials in an organized manner. https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1516972913959-9ZT3735DUX1DXNHC1KIZ/Screen+Shot+2018-01-25+at+2.58.27+PM.png Tutorials - Calibrating Optical Density IP Address: Modify the appropriate ip address for the eVOLVER you are trying to calibrate. To verify/ assign the ip address log into the router. Typically, most devices I've set up are set to 192.168.1.2 https://www.fynchbio.com/documentation daily 0.75 2023-04-17 https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1507701654793-66UOFTYCLWOLE2ZI46HU/IMG_7160.jpg Documentation Motherboard with Supplementary Boards https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1507701542006-2ZJFB3SJR7V6L9A2669C/IMG_7155.jpg Documentation Arduino Microcontroller https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1507701764048-9EKVW373BV8B1XNK46O8/IMG_7100.jpg Documentation Designed from DIY parts for continuous culture https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1507702514736-NSVAU86RX8LS0GX0CK6T/fynch_macicon.png Documentation https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1507702538423-Y2N9F8PWVW4OFZ9CIAAR/fynch_macicon.png Documentation https://images.squarespace-cdn.com/content/v1/5992045f2994ca7b03b75698/1507705249735-UQGPUTDWC3XPACFMZE5P/IMG_7239.jpg Documentation https://www.fynchbio.com/learn daily 0.75 2019-04-22